The PRPF39 Polyclonal Antibody (PAC061638) is a valuable tool for researchers studying the PRPF39 protein, which plays a crucial role in pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the PRPF39 protein, this antibody allows for the detection and analysis of PRPF39 in a variety of cell types, making it an ideal choice for investigations in molecular biology and gene expression studies.PRPF39 is essential for the splicing of precursor messenger RNA (pre-mRNA) into mature mRNA, a key step in gene expression regulation. Dysregulation of splicing processes can lead to various diseases, including cancer and genetic disorders.
Therefore, studying the function of PRPF39 is crucial for understanding how splicing abnormalities contribute to disease pathogenesis and for developing potential therapeutic interventions.This PAC061638 antibody provides researchers with a reliable tool to explore the role of PRPF39 in splicing mechanisms and its implications in disease development. By elucidating the functions of PRPF39, scientists can gain valuable insights into molecular processes and potentially identify new targets for therapeutic strategies in the treatment of various disorders related to aberrant RNA splicing.
IHC image of PACO61638 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with PACO61638 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO61638 diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PRPF39: Involved in pre-mRNA splicing. Belongs to the PRP39 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processingChromosomal Location of Human Ortholog: 14q21.2Cellular Component: snRNP U1Molecular Function: pre-mRNA 5'-splice site bindingBiological Process: nuclear mRNA 5'-splice site recognition
