The PRPF3 Polyclonal Antibody (CAB5482) is a valuable tool for researchers studying the PRPF3 protein, a critical component of the spliceosome complex involved in pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the PRPF3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential reagent for studies in molecular biology and genetic research.PRPF3's key role in the splicing machinery makes it a key player in the regulation of gene expression and the maintenance of cellular homeostasis.
Dysregulation of PRPF3 has been implicated in various human diseases, including neurodegenerative disorders and certain types of cancer. Investigating the function of PRPF3 is crucial for understanding its impact on cellular processes and identifying potential therapeutic targets for related pathologies. The PRPF3 Polyclonal Antibody is a valuable resource for advancing knowledge in the field of RNA splicing and gene regulation.
Product Name:
PRPF3 Rabbit Polyclonal Antibody
SKU:
CAB5482
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90; PRPF3
Positive Sample:
THP-1
Conjugate:
Unconjugated
Cellular Localization:
Nucleus speckle.
Calculated MW:
78kDa
Observed MW:
100kDa
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
Purification Method:
Affinity purification
Gene ID:
9129
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using PRPF3 Rabbit pAb (CAB5482) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
