The PRPF19 Polyclonal Antibody (CAB6706) is a valuable tool for research involving PRPF19, a key protein involved in pre-mRNA splicing. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunofluorescence. By specifically binding to the PRPF19 protein, this antibody allows for the detection and analysis of PRPF19 in different cell types, making it a powerful tool for studies in molecular biology and gene expression.PRPF19 is essential for the accurate splicing of precursor messenger RNA (pre-mRNA) into mature mRNA, a crucial step in gene expression regulation.
Dysregulation of pre-mRNA splicing has been implicated in various diseases, including cancer and genetic disorders. Therefore, understanding the functions and mechanisms of PRPF19 is important for unraveling the molecular basis of these conditions and developing targeted therapies.Overall, the PRPF19 Polyclonal Antibody (CAB6706) offers researchers a reliable means of studying PRPF19 and its role in pre-mRNA splicing, providing valuable insights into gene expression regulation and potential therapeutic targets in disease.
Product Name:
PRP19 Rabbit Polyclonal Antibody
SKU:
CAB6706
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRP19 (NP_055317.1).
Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.
Purification Method:
Affinity purification
Gene ID:
27339
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of SW620 cells, using PRP19 antibody (CAB6706) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
