The PRPF19 Polyclonal Antibody (CAB12590) is a valuable tool for researchers studying the PRPF19 protein, which is involved in pre-mRNA splicing in eukaryotic cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the PRPF19 protein, this antibody enables precise detection and analysis of PRPF19 in a variety of cell types, making it ideal for studies in molecular biology and genetic research.PRPF19 plays a crucial role in the spliceosome complex, which is responsible for removing introns from pre-mRNA to produce functional mRNA.
Dysregulation of PRPF19 has been linked to various diseases, including cancer and neurodegenerative disorders, making it an important target for therapeutic interventions. Understanding the function of PRPF19 is essential for unraveling the complexities of gene expression and splicing mechanisms, ultimately leading to the development of novel treatments for related diseases.
Product Name:
PRP19 Rabbit Polyclonal Antibody
SKU:
CAB12590
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRP19 (NP_055317.1).
Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.
Purification Method:
Affinity purification
Gene ID:
27339
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRP19 Rabbit pAb (CAB12590) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
