The PRPF18 Polyclonal Antibody (CAB12817) is a valuable tool for researchers studying PRPF18, a critical component of the spliceosome complex involved in pre-mRNA splicing. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PRPF18 protein, this antibody allows for the detection and analysis of PRPF18 in various cell types, making it essential for studies in molecular biology and RNA processing.PRPF18 plays a crucial role in the regulation of gene expression by ensuring accurate splicing of mRNA transcripts.
Dysregulation of PRPF18 has been linked to a variety of diseases, including cancer and genetic disorders. Understanding the function of PRPF18 is essential for unraveling the complexities of gene expression and developing targeted therapies for diseases associated with splicing defects. Researchers can rely on the PRPF18 Polyclonal Antibody to facilitate their investigations into this key molecular process.
Product Name:
PRPF18 Rabbit Polyclonal Antibody
SKU:
CAB12817
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1).
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.
Purification Method:
Affinity purification
Gene ID:
8559
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using PRPF18 Rabbit pAb (CAB12817) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
