The Protocadherin-15 Polyclonal Antibody (CAB10086) is a powerful tool for researchers studying Protocadherin-15, a cell adhesion molecule crucial for the development and function of sensory hair cells in the inner ear. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to Protocadherin-15, researchers can accurately detect and analyze this important protein in a variety of cell types, making it an excellent choice for studies in auditory development, sensory biology, and genetic disorders affecting the inner ear.
Protocadherin-15 is known for its role in forming the tip links that connect sensory hair cells in the inner ear, allowing for the detection of sound and motion. Mutations in the gene encoding Protocadherin-15 can lead to hearing loss, balance disorders, and other auditory abnormalities. By investigating the function of Protocadherin-15 using this antibody, researchers can gain valuable insights into the mechanisms underlying these disorders and potentially develop new therapeutic strategies for treating them.
Product Name:
PCDH15 Rabbit Polyclonal Antibody
SKU:
CAB10086
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human PCDH15 (NP_001136235.1).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
216kDa
Observed MW:
100kDa
This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur.
Purification Method:
Affinity purification
Gene ID:
65217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PCDH15 antibody (CAB10086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
