The WNT-5B Polyclonal Antibody (CAB8313) is a valuable tool for researchers studying the WNT-5B protein, a member of the WNT signaling pathway that plays a crucial role in cell growth, differentiation, and development. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications.WNT-5B is known to be involved in various biological processes, including embryogenesis, tissue homeostasis, and cancer progression. Its dysregulation has been linked to diseases such as cancer, cardiovascular disorders, and skeletal abnormalities.
By targeting the WNT-5B protein, researchers can gain insight into its functions and potential therapeutic interventions for related conditions.This polyclonal antibody enables the detection and analysis of WNT-5B in different cell types, allowing for a deeper understanding of its role in signal transduction and cellular processes. Its validation for Western blotting ensures reliable and accurate results for immunology and cancer research studies.
Product Name:
WNT5B Rabbit Polyclonal Antibody
SKU:
CAB8313
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human WNT5B (NP_110402.2).
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
Purification Method:
Affinity purification
Gene ID:
81029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse ovary, using WNT5B Rabbit pAb (CAB8313) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
