The WNT-5A Polyclonal Antibody (CAB2133) is a valuable tool for researchers studying the WNT-5A protein, a key player in the Wnt signaling pathway that regulates various cellular processes such as cell proliferation, differentiation, and migration. This antibody, generated in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications.WNT-5A is known to be involved in diverse biological functions, including embryonic development, tissue regeneration, and tumorigenesis. Dysregulation of the Wnt signaling pathway, particularly the aberrant expression of WNT-5A, has been linked to various diseases such as cancer, developmental disorders, and inflammatory conditions.
Understanding the role of WNT-5A in these pathological processes is essential for the development of targeted therapies and diagnostic tools.By utilizing the WNT-5A Polyclonal Antibody, researchers can accurately detect and analyze the expression levels of the WNT-5A protein in different cell types and tissues, providing valuable insights into its functions and potential therapeutic implications. This antibody is a valuable asset for studies in cancer biology, developmental biology, and regenerative medicine, offering a deeper understanding of the complex mechanisms underlying Wnt signaling and its impact on human health.
Product Name:
WNT5A Rabbit Polyclonal Antibody
SKU:
CAB2133
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 65-305 of human WNT5A (NP_003383.2).
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7474
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using WNT5A Rabbit pAb (CAB2133) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
