The Protein S100 B Polyclonal Antibody (CAB0676) is a valuable tool for researchers studying the S100 B protein, a member of the S100 family of calcium-binding proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the S100 B protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for research in neuroscience, cancer biology, and inflammatory diseases.S100 B is known to play a role in several cellular processes, including cell proliferation, differentiation, and apoptosis.
In the context of cancer, S100 B has been implicated in tumor growth, invasion, and metastasis, making it a promising target for cancer research. Furthermore, elevated levels of S100 B have been linked to neurodegenerative disorders and inflammatory conditions, highlighting the importance of studying this protein in various disease settings.By utilizing the Protein S100 B Polyclonal Antibody, researchers can gain insights into the functions and mechanisms of S100 B in health and disease, ultimately paving the way for the development of novel diagnostic tools and therapeutic strategies.
Product Name:
S100B Rabbit Polyclonal Antibody
SKU:
CAB0676
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B (NP_006263.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
NEF; S100; S100-B; S100beta; S100B
Positive Sample:
SK-MEL-28,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
11kDa
Observed MW:
11kDa
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Purification Method:
Affinity purification
Gene ID:
6285
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using S100B Rabbit pAb (CAB0676) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
