The Protein O-GlcNAcase Polyclonal Antibody (CAB9033) offered by Assay Genie is a valuable tool for researchers studying O-GlcNAcase, an enzyme involved in the removal of O-linked N-acetylglucosamine (O-GlcNAc) modifications on proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications.O-GlcNAcase plays a crucial role in cellular processes such as signal transduction, transcription, and metabolism by controlling the levels of O-GlcNAc modifications on key proteins. Dysregulation of O-GlcNAc modification has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.
Therefore, understanding the function of O-GlcNAcase is essential for unraveling the molecular mechanisms underlying these pathological conditions.By using the Protein O-GlcNAcase Polyclonal Antibody (CAB9033), researchers can detect and analyze O-GlcNAcase protein expression in different cell types, facilitating investigations into its physiological and pathological roles. This antibody is a valuable tool for studies in glycobiology, cell signaling, and disease mechanisms, providing valuable insights that may lead to the development of novel therapeutic strategies targeting O-GlcNAcase-related pathways.
Product Name:
MGEA5 Rabbit Polyclonal Antibody
SKU:
CAB9033
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).
Purification Method:
Affinity purification
Gene ID:
10724
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using MGEA5 Rabbit pAb (CAB9033) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
