The ERGIC-53 Polyclonal Antibody (CAB10440) is a valuable tool for researchers studying the ERGIC-53 protein, a key component of the early secretory pathway in cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.ERGIC-53 is an essential protein involved in the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus. Its role in protein trafficking and sorting makes it a critical player in the maintenance of cellular homeostasis and proper protein processing. The ERGIC-53 Polyclonal Antibody binds specifically to the ERGIC-53 protein, allowing for accurate detection and analysis in various cell types.
Research involving ERGIC-53 is crucial for understanding fundamental cellular processes and the mechanisms underlying protein trafficking defects in various diseases. This antibody serves as a reliable tool for investigating ERGIC-53 function and localization, making it a valuable asset for studies in cell biology, molecular biology, and disease pathology. By elucidating the role of ERGIC-53, researchers can gain insights into potential therapeutic targets for conditions linked to protein trafficking abnormalities.
Product Name:
LMAN1 Rabbit Polyclonal Antibody
SKU:
CAB10440
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1).
Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Single-pass membrane protein, Single-pass type I membrane protein.
Calculated MW:
54kDa
Observed MW:
55kDa
The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
Purification Method:
Affinity purification
Gene ID:
3998
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LMAN1 Rabbit pAb (CAB10440) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
