The CBFA2T2 Polyclonal Antibody (CAB7033) is a valuable tool for researchers studying the CBFA2T2 protein, also known as core-binding factor subunit alpha-2-like 2. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.CBFA2T2 is a transcriptional regulator that plays a crucial role in hematopoiesis and skeletal development. It is also implicated in leukemia and other cancers, making it a key target for cancer research. The CBFA2T2 Polyclonal Antibody binds specifically to the CBFA2T2 protein, allowing for accurate detection and analysis in various cell types.
By utilizing the CBFA2T2 Polyclonal Antibody in their studies, researchers can gain valuable insights into the function and regulation of CBFA2T2 in both normal and disease states. This antibody is a valuable tool for investigations into the molecular mechanisms underlying blood cell development and leukemia progression, providing a deeper understanding of these processes and potentially uncovering new therapeutic targets.
Product Name:
CBFA2T2 Rabbit Polyclonal Antibody
SKU:
CAB7033
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 325-604 of human CBFA2T2 (NP_005084.1).
In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
Purification Method:
Affinity purification
Gene ID:
9139
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CBFA2T2 antibody (CAB7033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
