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Protein Carbonyl Assay Kit - Colorimetric (MAES0096)

SKU:
MAES0096
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Hydrothorax, Cell Culture Supernatant, Tissue
Assay Time:
140 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
€409
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Protein Carbonyl Assay Kit - Colorimetric
Product Code:MAES0096
Product Size:96 Assays
Sample Type:Serum, plasma, hydrothorax, cell culture supernatant, tissue
Assay Time:140 min
Instrument:Microplate reader
Recovery Rate:97
Inter CV:8.5
Inter CV:5.2

The reactive oxygen species produced by aerobic metabolism in the body can cause the oxidation of DNA, lipids and proteins. The secondary reaction of the amino acid side chain of the protein with the lipid oxidation product is the main cause of the formation of the carbonyl. Carbonyl is a biological marker of ROS-mediated protein oxidation.

The content of protein carbonyl increases after oxidation, and the carbonyl group reacts with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Oxidative stress, Amino acids & proteins

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