The Proprotein Convertase 9 (PCSK9) Monoclonal Antibody is a vital tool for researchers studying PCSK9, an enzyme involved in regulating cholesterol levels in the body. This antibody, produced using hybridoma technology, specifically targets PCSK9 protein in human samples and is optimized for use in Western blot and immunohistochemistry applications.PCSK9 is known for its role in cholesterol metabolism, specifically by promoting the degradation of the LDL receptor, leading to increased levels of LDL cholesterol in the blood. Understanding the function of PCSK9 is crucial for developing therapies to lower cholesterol levels and reduce the risk of cardiovascular diseases.
This monoclonal antibody provides researchers with a reliable tool for detecting and studying PCSK9 in various cell types and tissues, making it ideal for investigations in cardiovascular health, lipid metabolism, and drug development. Its high specificity and sensitivity ensure accurate and reproducible results, advancing our understanding of PCSK9 biology and its implications for human health.
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
255738
Clone Number:
ARC0695
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Proprotein Convertase 9(PCSK9) Rabbit mAb (CAB21909) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
