The Progonadoliberin-2 Polyclonal Antibody (CAB8424) is a valuable tool for researchers studying Progonadoliberin-2, a key molecule involved in reproductive function and hormone regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to Progonadoliberin-2 protein, this antibody allows for the precise detection and analysis of Progonadoliberin-2 in a variety of cell types, making it ideal for investigations in the fields of endocrinology and reproductive biology.
Progonadoliberin-2, also known as GnRH-II, plays a crucial role in the regulation of gonadotropin-releasing hormone signaling, ultimately impacting processes such as ovulation, puberty, and fertility. Its involvement in the control of reproductive function makes it a target of interest in research related to reproductive disorders, infertility, and hormonal imbalances. By gaining a better understanding of the function of Progonadoliberin-2, researchers can identify potential therapeutic targets and develop treatments for conditions affecting the reproductive system.
Product Name:
GNRH2 Rabbit Polyclonal Antibody
SKU:
CAB8424
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human GNRH2 (NP_001492.1).
Sequence:
QHWS HGWY PGGK RALS SAQD PQNA LRPP GRAL DTAA GSPV QTAH GLPS DALA PLDD SMPW EGRT TAQW SLHR KRHL ARTL LTAA REPR PAPP SSNK V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
GnRH-II; LH-RHII; GNRH2
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
13kDa
Observed MW:
20kDa
This gene is a member of the gonadotropin-releasing hormone (GnRH) gene family. Proteins encoded by members of this gene family are proteolytically cleaved to form neuropeptides which, in part, regulate reproductive functions by stimulating the production and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The human GNRH2 gene is predicted to encode a preproprotein from which a mature neuropeptide of 10 amino acids is cleaved. However, while the human genome retains the sequence for a functional GNRH2 decapeptide, translation of the human GNRH2 gene has not yet been demonstrated and the GNRH2 gene of chimpanzees, gorilla, and Sumatran orangutan have a premature stop at codon eight of the decapeptide sequence which suggests GNRH2 was a pseudogene in the hominid lineage. The GNRH2 gene is also believed to be a pseudogene in many other mammalian species such as mouse and cow. The receptor for this gene (GNRHR2) is predicted to be a pseudogene in human as well as many other mammalian species. The closely related GNRH1 and GNRHR1 genes are functional in human and other mammals and are generally functional in vertebrates.
Purification Method:
Affinity purification
Gene ID:
2797
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using GNRH2 antibody (CAB8424) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
