The PRMT5 Polyclonal Antibody (CAB2290) is a valuable tool for research involving PRMT5, a protein involved in the regulation of gene expression and cellular processes. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the PRMT5 protein, researchers can detect and analyze its expression in various cell types, making it an essential reagent for studies in epigenetics, gene regulation, and cancer research.PRMT5, also known as protein arginine methyltransferase 5, is a key enzyme involved in the methylation of arginine residues in histones and other proteins, thereby impacting various cellular functions such as transcriptional regulation, RNA processing, and cell cycle progression.
Dysregulation of PRMT5 activity has been implicated in several diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Investigating the role of PRMT5 using this antibody is crucial for understanding its function and potential therapeutic implications in disease treatment.
Product Name:
PRMT5 Rabbit Polyclonal Antibody
SKU:
CAB2290
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 538-637 of human PRMT5 (NP_006100.2).
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10419
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PRMT5 antibody (CAB2290).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
