The PRKG2 Polyclonal Antibody (CAB18575) is a vital tool for research involving PRKG2, a protein kinase G specifically localized in the nucleus, regulating numerous cellular processes. This antibody, derived from rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PRKG2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it particularly suitable for studies in molecular biology and signal transduction pathways.
PRKG2, also known as cGMP-dependent protein kinase 2, is a key player in mediating signal transduction in response to intracellular cGMP levels. Its role in regulating processes such as cell growth, differentiation, and survival underscores its importance in various physiological and pathological conditions. Research on PRKG2 is essential for gaining insights into diseases like cancer, cardiovascular disorders, and neurological conditions, paving the way for potential therapeutic interventions targeting this critical signaling molecule.
Product Name:
PRKG2 Rabbit Polyclonal Antibody
SKU:
CAB18575
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PRKG2 (NP_006250.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
AMD4; PKG2; SMDP; cGK2; cGKII; PRKGR2; PRKG2
Positive Sample:
Mouse lung,Rat brain
Cellular Localization:
Apical cell membrane, Lipid-anchor.
Calculated MW:
87kDa
Observed MW:
100kDa
This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical.
Purification Method:
Affinity purification
Gene ID:
5593
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRKG2 Rabbit pAb (CAB18575) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
