The PRKG1 Polyclonal Antibody (CAB2565) is a versatile tool for researchers studying the protein kinase PRKG1, a key regulator of smooth muscle relaxation and vasodilation. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it a reliable choice for Western blot applications.PRKG1, also known as cGMP-dependent protein kinase 1, plays a crucial role in various physiological processes such as regulating blood flow, platelet function, and smooth muscle contraction. Dysregulation of PRKG1 activity has been implicated in cardiovascular diseases, erectile dysfunction, and certain types of cancer, highlighting its importance as a therapeutic target.
With the PRKG1 Polyclonal Antibody, researchers can accurately detect and analyze PRKG1 protein levels in a variety of cell types, enabling deeper insights into its function and potential implications for disease pathology. This antibody is a valuable tool for studies in cardiovascular biology, pharmacology, and drug development efforts aimed at targeting PRKG1 for therapeutic purposes.
Product Name:
PRKG1 Rabbit Polyclonal Antibody
SKU:
CAB2565
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKG1 (NP_006249.1).
Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.
Purification Method:
Affinity purification
Gene ID:
5592
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HepG2 cells, using PRKG1 antibody (CAB2565) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).
