The PRKDC Polyclonal Antibody (CAB1419) is developed for research involving PRKDC, a protein involved in DNA repair and maintenance of genomic stability. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blotting. It specifically targets the PRKDC protein, allowing for reliable detection and analysis in a wide range of cell types, making it an ideal tool for studies in molecular biology and cancer research.PRKDC, also known as DNA-PKcs, is a key player in the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair, which is crucial for preserving genetic integrity.
Dysregulation of PRKDC has been linked to various diseases, including cancer and genetic disorders, highlighting its importance in cellular homeostasis and disease development. Understanding the function and regulation of PRKDC is essential for developing targeted therapies and interventions in these pathological contexts.
Product Name:
PRKDC Rabbit Polyclonal Antibody
SKU:
CAB1419
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 3993-4128 of human DNA-PKcs (NP_008835.5).
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Purification Method:
Affinity purification
Gene ID:
5591
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNA-PKcs Rabbit pAb (CAB1419) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
