The PRKCZ Polyclonal Antibody (CAB5714) is a valuable tool for researchers studying PRKCZ, a protein kinase C (PKC) family member involved in various cellular processes, including cell proliferation, differentiation, and apoptosis. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. PRKCZ is known to play a crucial role in signaling pathways that regulate cell growth and survival, making it a potential target for cancer research and drug development.
By targeting PRKCZ with this antibody, researchers can better understand its function and potential therapeutic implications in cancer and other diseases. With its proven reliability and efficacy in detecting PRKCZ protein, the PRKCZ Polyclonal Antibody (CAB5714) is a valuable asset for researchers studying the intricate mechanisms of cellular signaling and potential therapeutic targets in various diseases.
Product Name:
PKC zeta Rabbit Polyclonal Antibody
SKU:
CAB5714
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 476-547 of human PKC zeta (NP_002735.3).
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5590
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PKC zeta antibody (CAB5714) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
