The PRKCI Polyclonal Antibody (CAB18348) is a valuable tool for researchers studying PRKCI, a protein kinase C involved in various signaling pathways and cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards PRKCI in human samples, making it suitable for use in Western blot assays.PRKCI, also known as Protein Kinase C iota, is a key regulator of cell proliferation, survival, and migration, and is implicated in cancer development and progression.
By targeting PRKCI with this antibody, researchers can analyze its expression levels and distribution in different cell types, shedding light on its functional role in cancer biology. This antibody is an essential resource for investigations into PRKCI-mediated signaling pathways and their potential as therapeutic targets in oncology research.
Product Name:
PRKCI Rabbit Polyclonal Antibody
SKU:
CAB18348
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-596 of human PRKCI (P41743).
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
Purification Method:
Affinity purification
Gene ID:
5584
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRKCI Rabbit pAb (CAB18348) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
