The Anti-PRKAR2B Antibody (CAB19745) is a polyclonal antibody designed for research involving PRKAR2B, a key regulatory subunit of protein kinase A (PKA) involved in various signaling pathways. The antibody, raised in rabbits, is highly specific and reacts with human PRKAR2B samples, making it ideal for use in Western blot and immunohistochemistry applications. PRKAR2B plays a crucial role in cellular signaling, particularly in regulating processes such as cell growth, metabolism, and gene expression. Dysregulation of PKA signaling has been implicated in a variety of diseases, including cancer, cardiovascular disorders, and neurological conditions.
Studying the function of PRKAR2B can provide valuable insights into these disease mechanisms and potential therapeutic targets. By targeting PRKAR2B, researchers can explore its role in various cellular processes and disease states, ultimately leading to a better understanding of its function and potential implications for drug development and treatment strategies.
Product Name:
PRKAR2B Rabbit Monoclonal Antibody
SKU:
CAB19745
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 319-418 of human PRKAR2B (P31323).
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Purification Method:
Affinity purification
Gene ID:
5577
Clone Number:
ARC2272
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRKAR2B Rabbit mAb (CAB19745) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
