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[KO Validated] PKA RIIα (PRKAR2A) Rabbit Polyclonal Antibody (CAB1531)

SKU:
CAB1531
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-PRKAR2A Antibody CAB1531KO Validated
  • KO Validated Antibodies 1 Anti-PRKAR2A Antibody CAB1531KO Validated
  • KO Validated Antibodies 1 Anti-PRKAR2A Antibody CAB1531KO Validated
€139 - €419
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Description

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Product Name:[KO Validated] PKA RIIα (PRKAR2A) Rabbit Polyclonal Antibody
SKU:CAB1531
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RIIα (PRKAR2A)/PKR2 (NP_004148.1).
Sequence:MSHI QIPP GLTE LLQG YTVE VLRQ QPPD LVEF AVEY FTRL REAR APAS VLPA ATPR QSLG HPPP EPGP DRVA DAKG DSES EEDE DLEV PVPS RFNR RVSV CAET YNPD EEEE DTDP RVIH PKTD EQRC RLQE ACKD ILLF KNLD QEQL SQVL DAMF ERIV KADE HVID QGDD GDNF YVIE RGTY DILV TKDN QTRS VGQY DNRG SFGE LALM YNTP RAAT IVAT SEGS LWGL DRVT FRRI IVKN NAKK RKMF ESFI ESVP LLKS LEVS ERMK IVDV IGEK IYKD GERI ITQG EKAD SFYI IESG EVSI LIRS RTKS NKDG GNQE VEIA RCHK GQYF GELA LVTN KPRA ASAY AVGD VKCL VMDV QAFE RLLG PCMD IMKR NISH YEEQ LVKM FGSS VDLG NLGQ
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:PKR2; PRKAR2; A)
Positive Sample:HeLa,Jurkat,SW480,LO2,Mouse lung,Rat skeletal muscle
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:46kDa
Observed MW:46kDa

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

Purification Method:Affinity purification
Gene ID:5576
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using PKA RIIα (PRKAR2A)/PKR2 antibody (CAB1531) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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