The PRKAR1B Polyclonal Antibody (CAB10125) is a valuable tool for researchers studying PRKAR1B, a protein kinase involved in cell signaling and gene expression regulation. This antibody, produced through rabbit immunization, shows high reactivity with human samples and is suitable for Western blot analyses. By specifically binding to the PRKAR1B protein, this antibody enables the detection and examination of PRKAR1B in various cell types, making it ideal for investigations in molecular biology and cancer research.
PRKAR1B, also known as cAMP-dependent protein kinase type I-beta regulatory subunit, plays a crucial role in mediating cellular responses to various stimuli, including hormones and neurotransmitters. Its involvement in signaling pathways makes it an attractive target for studies on cancer, metabolic disorders, and neurological diseases. Understanding the function of PRKAR1B is essential for developing therapies that target its activity and impact cellular processes in disease contexts.
Product Name:
PRKAR1B Rabbit Polyclonal Antibody
SKU:
CAB10125
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1).
The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5575
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PRKAR1B antibody (CAB10125) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
