The PRKAR1A Polyclonal Antibody (CAB0906) is a valuable tool for researchers studying PRKAR1A, a key regulatory subunit of protein kinase A (PKA) involved in various cellular processes such as cell growth, metabolism, and gene expression. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the PRKAR1A protein, this antibody allows for the detection and analysis of PRKAR1A levels in different cell types, making it ideal for studies in cancer research and metabolic disorders.
PRKAR1A plays a crucial role in modulating PKA activity, which is essential for regulating various signaling pathways involved in cell proliferation and survival. Dysregulation of PRKAR1A has been linked to tumorigenesis and other diseases, making it a promising target for therapeutic interventions. Understanding the functions and mechanisms of PRKAR1A can provide insights into potential treatment strategies for cancer and metabolic disorders, ultimately contributing to the development of new therapeutic approaches in the field of biomedicine.
Product Name:
PRKAR1A Rabbit Polyclonal Antibody
SKU:
CAB0906
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PRKAR1A (NP_002725.1).
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5573
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] PRKAR1A Rabbit pAb (CAB0906) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
