The PRKACA, PRKACB, and PRKACG Polyclonal Antibody (PACO06760) is a valuable tool for researchers studying protein kinase A catalytic subunits. These subunits play essential roles in various signaling pathways, including those involved in cellular growth, metabolism, and gene expression.Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting PRKACA, PRKACB, and PRKACG proteins, researchers can accurately detect and analyze these critical molecules in a variety of cell types.The PRKACA, PRKACB, and PRKACG Polyclonal Antibody is particularly useful in immunology and cancer research, where dysregulation of protein kinase A activity is often observed.
By understanding the functions and interactions of these catalytic subunits, researchers can uncover new insights into disease mechanisms and potentially identify novel therapeutic targets for conditions such as cancer, metabolic disorders, and cardiovascular diseases.Overall, the PRKACA, PRKACB, and PRKACG Polyclonal Antibody is a valuable resource for researchers seeking to explore the complex roles of protein kinase A catalytic subunits in various cellular processes and disease states.
Synthesized peptide derived from human PKA α / β / γ cat around the non-phosphorylation site of T198.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of 22RV1 HELA cells using PKA alpha / beta / gamma cat Polyclonal Antibody.
Western Blot analysis of COLO205 cells using PKAalpha / beta / gamma cat Polyclonal Antibody.
Synonyms:
PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit α; PKA C-α; PRKACB; cAMP-dependent protein kinase catalytic subunit β; PKA C-β; PRKACG; cAMP-dependent protein kinase catalytic subunit γ; PKA C-γ
UniProt Protein Function:
PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.
UniProt Protein Details:
Protein type:Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; AGC group; PKA family
Chromosomal Location of Human Ortholog: 19p13.1
Cellular Component: centrosome; membrane; mitochondrion; plasma membrane; cytosol; nucleus; neuromuscular junction; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex
Molecular Function:protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding
Biological Process: nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; water transport; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; regulation of synaptic transmission; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytosolic calcium ion homeostasis; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; neural tube closure; carbohydrate metabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; regulation of insulin secretion
NCBI Summary:
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
