The PRKAA2 Polyclonal Antibody (CAB7339) is a valuable tool for studying the PRKAA2 protein, a key regulator of cellular energy metabolism and autophagy. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting PRKAA2 in human samples, making it suitable for use in Western blotting applications. By binding to PRKAA2, this antibody enables the accurate detection and analysis of the protein in a variety of cell types, offering researchers a reliable tool for investigations in the fields of metabolism, energy regulation, and autophagy.
PRKAA2, also known as AMP-activated protein kinase alpha 2 subunit, plays a crucial role in maintaining cellular energy homeostasis by sensing and responding to fluctuations in energy levels. Dysregulation of PRKAA2 has been implicated in various metabolic disorders, including diabetes, obesity, and cancer, highlighting its significance as a potential therapeutic target. Studying the function and regulation of PRKAA2 using this antibody may provide valuable insights into the development of novel treatments for metabolic diseases and cancer.
Product Name:
[KO Validated] AMPKα2 Rabbit Polyclonal Antibody
SKU:
CAB7339
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-343 of human AMPKα2 (NP_006243.2).
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Purification Method:
Affinity purification
Gene ID:
5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver, using [KO Validated] AMPKα2 Rabbit pAb (CAB7339) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
