The PRKAA2 Polyclonal Antibody (CAB14052) is a valuable tool for researchers studying PRKAA2, a key regulator of cellular energy metabolism and a critical component of the AMP-activated protein kinase (AMPK) pathway. This antibody is produced in rabbits and is highly specific for human samples, making it ideal for Western blot applications.PRKAA2, also known as AMPKα2, is involved in the regulation of cellular energy homeostasis and metabolism. It plays a crucial role in activating catabolic pathways to generate ATP and in inhibiting anabolic pathways during times of cellular stress.
The PRKAA2 Polyclonal Antibody enables the detection and analysis of PRKAA2 protein levels, allowing researchers to study its function in various cell types.By understanding the activity of PRKAA2, researchers can gain insights into how cellular energy metabolism is regulated and how dysregulation of this pathway may contribute to diseases such as cancer, metabolic disorders, and neurodegenerative conditions. The PRKAA2 Polyclonal Antibody is a valuable tool for investigating the role of PRKAA2 in these disease contexts and for developing potential therapies targeting this pathway.
Product Name:
AMPKα2 Rabbit Polyclonal Antibody
SKU:
CAB14052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-343 of human AMPKα2 (NP_006243.2).
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Purification Method:
Affinity purification
Gene ID:
5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PRKAA2 antibody (CAB14052) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
