The PRKAA1 Polyclonal Antibody (CAB16960) is specifically designed for research involving PRKAA1, a key protein involved in regulating cellular energy metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to PRKAA1, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential tool for studies in cell biology, metabolism, and cancer research.PRKAA1, also known as AMP-activated protein kinase (AMPK) alpha 1, plays a crucial role in maintaining cellular energy homeostasis by sensing and responding to changes in AMP/ATP ratios.
Dysregulation of PRKAA1 activity has been implicated in various metabolic disorders, including diabetes, obesity, and cancer. Therefore, understanding the function and regulation of PRKAA1 is vital for developing targeted therapies aimed at restoring energy balance and combating metabolic diseases.Overall, the PRKAA1 Polyclonal Antibody (CAB16960) offers researchers a reliable tool for studying the role of PRKAA1 in cellular metabolism and its potential as a therapeutic target in metabolic diseases and cancer.
Product Name:
AMPKα1 Rabbit Polyclonal Antibody
SKU:
CAB16960
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 421-520 of human AMPKα1 (NP_006242.5).
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AMPKα1 Rabbit pAb (CAB16960) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
