The PRIMPOL Antibody (PAC046834) is a valuable tool for researchers studying PRIMPOL, a protein involved in DNA replication and repair processes. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the PRIMPOL protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.PRIMPOL plays a crucial role in maintaining genomic stability by promoting accurate DNA replication and repair mechanisms.
Dysregulation of PRIMPOL activity has been linked to various diseases, including cancer and genetic disorders. Therefore, understanding the function of PRIMPOL is crucial for developing targeted therapies and diagnostic tools for these conditions. The PRIMPOL Antibody offers researchers a reliable method to explore the complex interactions of PRIMPOL in cellular processes, advancing our understanding of its role in health and disease.
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO46834 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO46834 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
DNA primase and DNA polymerase able to initiate de novo DNA synthesis using dNTPs. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Involved in translesion synthesis via its primase activity by mediating uninterrupted fork progression after programmed or damage-induced fork arrest and by reinitiating DNA synthesis after dNTP depletion. Required for mitochondrial DNA (mtDNA) synthesis, suggesting it may be involved in DNA tolerance during the replication of mitochondrial DNA. Has non-overlapping function with POLH.
Synonyms:
DNA-directed primase/polymerase protein (hPrimpol1) (EC 2.7.7) (Coiled-coil domain-containing protein 111), PRIMPOL, CCDC111
UniProt Protein Function:
PRIMPOL: DNA primase and DNA polymerase able to initiate de novo DNA synthesis using dNTPs. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Involved in translesion synthesis via its primase activity by mediating uninterrupted fork progression after programmed or damage-induced fork arrest and by reinitiating DNA synthesis after dNTP depletion. Required for mitochondrial DNA (mtDNA) synthesis, suggesting it may be involved in DNA tolerance during the replication of mitochondrial DNA. Has non-overlapping function with POLH. Belongs to the eukaryotic-type primase small subunit family. Interacts with RPA1. 2 isoforms of the human protein are produced by alternative splicingProtein type: DNA repair, damage; DNA replication; MitochondrialChromosomal Location of Human Ortholog: 4q35.1Cellular Component: mitochondrial matrix; nucleusMolecular Function: chromatin binding; DNA primase activity; DNA-directed DNA polymerase activity; manganese ion binding; protein bindingBiological Process: bypass DNA synthesis; mitochondrial DNA replication; replication fork processing; response to UVDisease: Myopia 22, Autosomal Dominant
