The PRIM1 Polyclonal Antibody (CAB16959) is a valuable tool for researchers studying PRIM1, a protein involved in DNA replication. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications. By binding to the PRIM1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and cancer research.
PRIM1 plays a crucial role in DNA replication by facilitating the synthesis of primer RNA, a key step in the initiation of DNA synthesis. Dysregulation of PRIM1 has been implicated in a variety of cancers and genetic disorders, making it a promising target for therapeutic interventions. By studying PRIM1 activity, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new strategies for treatment.
Product Name:
PRIM1 Rabbit Polyclonal Antibody
SKU:
CAB16959
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 121-420 of human PRIM1 (NP_000937.1).
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
Purification Method:
Affinity purification
Gene ID:
5557
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using PRIM1 Rabbit pAb (CAB16706) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
