The Prickle3 Polyclonal Antibody (PAC061959) is a valuable tool for researchers studying Prickle3, a protein involved in cellular signaling pathways and development. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Prickle3 protein, researchers can easily detect and analyze its expression in a variety of cell types.Prickle3 is known for its role in controlling cell polarity and tissue morphogenesis, making it a key player in developmental biology and cancer research.
Understanding the function of Prickle3 can provide insights into how cells organize themselves and how disruptions in these processes can contribute to diseases like cancer. By targeting Prickle3, researchers can potentially uncover new therapeutic approaches for treating various diseases.Overall, the Prickle3 Polyclonal Antibody is a valuable tool for researchers interested in studying cellular signaling, development, and disease mechanisms. Its specificity and high reactivity make it ideal for a wide range of experimental applications in both basic and clinical research settings.
Western Blot. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate. All lanes: PRICKLE3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 60 kDa. Observed band size: 69 kDa.
IHC image of PACO61959 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation (By similarity). PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module (By similarity). Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions (By similarity). Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity).
Synonyms:
Prickle planar cell polarity protein 3 (LIM domain only protein 6) (LMO-6) (Prickle-like protein 3) (Pk3) (Triple LIM domain protein 6), PRICKLE3, LMO6
UniProt Protein Function:
LMO6: LIM domain only 6 is a three LIM domain-containing protein. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface for protein-protein interactions. [provided by RefSeq, Jul 2008]Chromosomal Location of Human Ortholog: Xp11.23Molecular Function: protein binding
UniProt Protein Details:
NCBI Summary:
LIM domain only 6 is a three LIM domain-containing protein. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface for protein-protein interactions. [provided by RefSeq, Jul 2008]
