PREX2 Antibody (PACO47946)
- SKU:
- PACO47946
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
PREX2 Antibody (PACO47946)
The PREX2 Antibody (PAC047946) is a polyclonal antibody designed for research involving PREX2, a protein that plays a key role in cell signaling and regulation. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting and immunohistochemistry.PREX2 is a guanine nucleotide exchange factor that activates the PI3K signaling pathway, leading to cell proliferation and survival. Dysregulation of PREX2 has been implicated in various diseases, including cancer, making it an important target for research in oncology and cell biology.
The PREX2 Antibody enables the detection and analysis of PREX2 protein levels in different cell types, providing valuable insights into its function and potential as a therapeutic target.Understanding the role of PREX2 in cell signaling pathways is essential for developing targeted therapies for cancer and other diseases characterized by abnormal cell growth and survival. The PREX2 Antibody is a valuable tool for researchers investigating the mechanisms underlying these conditions and developing new treatment strategies.
| Antibody Name: | PREX2 Antibody (PACO47946) |
| Antibody SKU: | PACO47946 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (1417-1592AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: PREX2 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 183, 172, 113 kDa. Observed band size: 183, 113 kDa.. |
 | Immunofluorescent analysis of HepG2 cells using PACO47946 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO47946 at dilution of 1:100. |
| Background: | Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the β γ subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase. |
| Synonyms: | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (P-Rex2) (PtdIns(3,4,5)-dependent Rac exchanger 2) (DEP domain-containing protein 2), PREX2, DEPDC2 |
| UniProt Protein Function: | PREX2: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Rac/Rho; Phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 8q13.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:GTPase activator activity; Rac guanyl-nucleotide exchange factor activity Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of protein kinase activity; negative regulation of TOR signaling pathway |
| NCBI Summary: | The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016] |
| UniProt Code: | Q70Z35 |
| NCBI GenInfo Identifier: | 47578115 |
| NCBI Gene ID: | 80243 |
| NCBI Accession: | NP_079146.2 |
| UniProt Secondary Accession: | Q70Z35,Q32KL0, Q32KL1, Q6R7Q3, Q6R7Q4, Q9H805, Q9H961 B4DFX0, |
| UniProt Related Accession: | Q70Z35 |
| Molecular Weight: | |
| NCBI Full Name: | phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform a |
| NCBI Synonym Full Names: | phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 |
| NCBI Official Symbol: | PREX2Â Â |
| NCBI Official Synonym Symbols: | DEP.2; DEPDC2; P-REX2; PPP1R129Â Â |
| NCBI Protein Information: | phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
| UniProt Protein Name: | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
| UniProt Synonym Protein Names: | DEP domain-containing protein 2 |
| Protein Family: | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
| UniProt Gene Name: | PREX2Â Â |
| UniProt Entry Name: | PREX2_HUMAN |
| ELISA Kits |
| Human PREX2 ELISA Kit |
| Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Human PREX2 ELISA Kit
Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit (HUEB2437)
Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (Prex2) ELISA Kit (MOEB2187)
