The Pregnenolone ELISA Kit from Assay Genie is a highly reliable and accurate tool for measuring pregnenolone levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Pregnenolone is a key neurosteroid involved in various physiological processes, including memory and cognition, mood regulation, and stress response.
Dysregulation of pregnenolone levels has been associated with conditions such as Alzheimer's disease, depression, and anxiety disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and high-quality components, the Pregnenolone ELISA Kit is an essential tool for researchers and clinicians alike seeking to investigate the role of pregnenolone in health and disease.
Product Name:
Pregnenolone ELISA Kit (UNES00054)
SKU:
UNES00054
Target:
Pregnenolone ELISA Kit (UNES00054)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with P5. During the reaction, P5 in the sample or standard competes with a fixed amount of P5 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to P5. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P5 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
93-110
84-98
Average (%)
96
101
90
1:4
Range (%)
94-106
92-106
86-99
Average (%)
101
99
92
1:8
Range (%)
101-116
94-105
87-100
Average (%)
107
99
93
1:16
Range (%)
94-111
87-102
91-102
Average (%)
101
94
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.9
548.1
1704.4
189.7
527.6
1854.8
Standard deviation
12
25.8
56.2
12.5
23.7
96.4
C V (%)
5.8
4.71
3.3
6.59
4.49
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of P5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes P5 in samples. No significant cross-reactivity or interference between P5 and analogues was observed.
