The PRDX5 Polyclonal Antibody (CAB6329) is a valuable tool for researchers studying PRDX5, a peroxiredoxin protein involved in antioxidant defense and redox regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding to the PRDX5 protein, this antibody enables detection and analysis in a variety of cell types, making it ideal for studies in oxidative stress, cancer biology, and redox signaling pathways.PRDX5, also known as peroxisomal antioxidant enzyme, plays a crucial role in maintaining cellular redox balance and protecting cells from oxidative damage.
Its involvement in regulating reactive oxygen species levels links it to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Investigating the functions and mechanisms of PRDX5 can provide insights into disease pathogenesis and identify potential therapeutic targets for oxidative stress-related conditions.
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene.
Purification Method:
Affinity purification
Gene ID:
25824
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (Peroxiredoxin 5 (PRDX5)) antibody (CAB6329) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
