The PRDX5 Polyclonal Antibody (CAB1269) is specifically designed for studies involving peroxiredoxin-5 (PRDX5), a vital antioxidant enzyme located in the mitochondria. This antibody is produced in rabbits and has a high reactivity with human samples, making it well-suited for use in Western blot applications. By binding to the PRDX5 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for research in oxidative stress, aging, and neurodegenerative diseases.PRDX5 is known for its crucial role in protecting cells from oxidative damage and maintaining redox homeostasis in the mitochondria.
Its function as an antioxidant enzyme makes it a key player in defending against reactive oxygen species and maintaining cellular health. Research on PRDX5 is essential for understanding its contribution to various diseases, such as cancer, cardiovascular diseases, and neurodegenerative disorders, where oxidative stress plays a significant role.By utilizing the PRDX5 Polyclonal Antibody (CAB1269), researchers can gain valuable insights into the role of PRDX5 in cellular protection and disease development, paving the way for potential therapeutic strategies targeting oxidative stress-related conditions.
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene.
Purification Method:
Affinity purification
Gene ID:
25824
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (Peroxiredoxin 5 (PRDX5)) antibody (CAB1269) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
