The PRDX4 Polyclonal Antibody (CAB18308) is specifically designed for research involving PRDX4, a protein implicated in antioxidant defense and cell survival. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the PRDX4 protein, allowing for precise detection and analysis in various cell types, making it an ideal tool for studies in oxidative stress, cancer research, and cellular signaling pathways.
PRDX4, also known as peroxiredoxin-4, plays a crucial role in protecting cells from oxidative damage and promoting cell survival. Its involvement in redox regulation makes it a key player in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. Understanding the function of PRDX4 is essential for developing therapeutic strategies that target oxidative stress-related conditions and improve overall cellular health.
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Purification Method:
Affinity purification
Gene ID:
10549
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) Rabbit pAb (CAB18308) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
