The PRDM14 Polyclonal Antibody (CAB6502) is a crucial tool for research involving the PRDM14 protein, a key regulator of stem cell pluripotency and embryonic development. This antibody, derived from rabbit hosts, is highly specific and reactive with human samples, making it an ideal choice for a variety of applications, including Western blotting.PRDM14 is a transcription factor that plays a pivotal role in maintaining stem cell properties and regulating embryonic development. By targeting PRDM14, researchers can gain valuable insights into its function and its impact on various cellular processes.
This antibody enables accurate detection and analysis of PRDM14 expression, making it invaluable for studies in stem cell biology, developmental biology, and regenerative medicine.The PRDM14 Polyclonal Antibody is a versatile tool that can help researchers deepen their understanding of the mechanisms underlying stem cell pluripotency and embryonic development. Its high specificity and reactivity make it a valuable asset for investigations into the role of PRDM14 in various biological processes, paving the way for potential therapeutic applications in regenerative medicine and disease treatment.
Antibody Name:
Anti-PRDM14 Antibody
Antibody SKU:
CAB6502
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant protein of mouse PRDM14
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
Mouse liver
Immunogen:
Recombinant protein of mouse PRDM14
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
383491
Uniprot:
Cellular Location:
Nucleus
Calculated MW:
64kDa
Observed MW:
64kDa
Synonyms:
PRDM14, PFM11
Background:
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Western blot analysis of extracts of Mouse liver, using PRDM14 antibody (CAB6502) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Immunofluorescence analysis of HeLa cells using PRDM14 antibody (CAB6502) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody (CAB6502) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using PRDM14 antibody (CAB6502) at dilution of 1:100. Blue: DAPI for nuclear staining.
