The PRDM14 Polyclonal Antibody (CAB5543) is a crucial tool for researchers studying the PRDM14 protein, a key player in stem cell pluripotency and cancer development. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the PRDM14 protein, this antibody enables the detection and analysis of PRDM14 in various cell types, making it an ideal choice for studies in stem cell biology and cancer research.PRDM14 is a transcription factor that plays a crucial role in maintaining the pluripotency of embryonic stem cells and induced pluripotent stem cells.
Additionally, PRDM14 has been implicated in the development and progression of various types of cancer, making it a promising target for therapeutic interventions. Understanding the functions and regulatory mechanisms of PRDM14 is essential for advancing our knowledge of stem cell biology and cancer biology, ultimately paving the way for the development of novel treatments for cancer patients.
Product Name:
PRDM14 Rabbit Polyclonal Antibody
SKU:
CAB5543
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRDM14 (NP_078780.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
PFM11; PRDM14
Positive Sample:
NCI-H125,Mouse ovary,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
64kDa
Observed MW:
70kDa
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Purification Method:
Affinity purification
Gene ID:
63978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRDM14 Rabbit pAb (CAB5543) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
