The PRC1 Polyclonal Antibody (CAB7029) is a crucial tool for researchers studying the protein PRC1, which is involved in regulating cell division and chromosome segregation during mitosis. This antibody, generated in rabbits, has been optimized for use in Western blot applications and demonstrates high specificity and sensitivity when detecting PRC1 in human samples.PRC1 plays a vital role in ensuring accurate chromosome segregation during cell division, making it a key target for research in cancer biology and cell cycle regulation. Dysregulation of PRC1 expression or function has been associated with various types of cancer, making it an important focus for understanding tumor development and progression.
By using the PRC1 Polyclonal Antibody, researchers can gain valuable insights into the role of PRC1 in cell division, chromosome segregation, and its implications in cancer biology. This antibody is an essential tool for studies aiming to elucidate the molecular mechanisms underlying cell cycle regulation and identify potential therapeutic targets for cancer treatment.
Product Name:
PRC1 Rabbit Polyclonal Antibody
SKU:
CAB7029
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 351-620 of human PRC1 (NP_003972.1).
Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle pole.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9055
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRC1 Rabbit pAb (CAB7029) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
