The PRAME Polyclonal Antibody (CAB14507) is a valuable tool for researchers studying PRAME, a tumor-associated antigen commonly found in various cancer types. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically targets the PRAME protein, allowing for accurate detection and analysis in a variety of cell types typically found in cancer research.PRAME, or Preferentially Expressed Antigen in Melanoma, is involved in promoting tumor growth and survival, making it a potential target for cancer therapy development.
Understanding the role of PRAME in tumor progression is essential for identifying new treatment strategies for cancer patients. The PRAME Polyclonal Antibody provides a reliable tool for investigating the expression and function of PRAME in tumor cells, supporting research efforts in oncology and immunotherapy.
Product Name:
PRAME Rabbit Polyclonal Antibody
SKU:
CAB14507
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1).
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
23532
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRAME Rabbit pAb (CAB14507) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
