The PPP4R1 Polyclonal Antibody (CAB8361) is a powerful tool for researchers studying PPP4R1, a regulatory subunit of protein phosphatase 4 (PPP4). This antibody, produced in rabbits, has high specificity and sensitivity for detecting PPP4R1 in human samples, making it an ideal choice for Western blot applications.PPP4R1 is a key player in cellular processes such as cell cycle regulation, DNA damage repair, and gene transcription. Dysregulation of PPP4R1 has been associated with various diseases, including cancer and neurodegenerative disorders.
By targeting PPP4R1 with this antibody, researchers can explore its role in these diseases and potentially uncover new therapeutic targets.With its ability to specifically bind to PPP4R1, the CAB8361 antibody enables detailed analysis of PPP4R1 expression levels and localization in different cell types. This antibody is a valuable tool for scientists investigating the molecular mechanisms underlying disease pathology and seeking to develop targeted treatments.
Product Name:
PPP4R1 Rabbit Polyclonal Antibody
SKU:
CAB8361
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MEG1; PP4R1; PP4(Rmeg); PPP4R1
Positive Sample:
293T,U-251MG,BT-474,K-562,SKOV3
Conjugate:
Unconjugated
Calculated MW:
107kDa
Observed MW:
130kDa
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9989
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPP4R1 Rabbit pAb (CAB8361) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
