The PPP3R1 Polyclonal Antibody (CAB0954) is a vital tool for researchers studying PPP3R1, a key regulatory subunit of the protein phosphatase 3 (PPP3) complex. This antibody, produced in rabbits, shows strong reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the PPP3R1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and signal transduction pathways.
PPP3R1, also known as calcineurin B-like protein 1 (CBL), plays a crucial role in cell signaling processes, including cell growth, differentiation, and apoptosis. Dysregulation of PPP3R1 has been linked to various diseases, such as cancer, neurological disorders, and autoimmune conditions. Understanding the function and regulation of PPP3R1 is essential for developing targeted therapies that could potentially intervene in these disease pathways and improve patient outcomes.
Product Name:
PPP3R1 Rabbit Polyclonal Antibody
SKU:
CAB0954
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_000936.1).
Enables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimer's disease and dilated cardiomyopathy.
Purification Method:
Affinity purification
Gene ID:
5534
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PPP3R1 antibody (CAB0954) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
