The PPP1R15A Polyclonal Antibody (CAB16260) is a valuable tool for researchers studying PPP1R15A, a protein involved in the regulation of cellular stress responses. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PPP1R15A protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology and molecular biology research.PPP1R15A, also known as GADD34, is a key player in the unfolded protein response pathway, which is activated in response to cellular stress such as endoplasmic reticulum (ER) stress.
Dysregulation of this pathway has been implicated in various diseases including neurodegenerative disorders, cancer, and metabolic syndromes. Research into the function and regulation of PPP1R15A is crucial for understanding its role in cellular stress responses and its potential as a therapeutic target for these diseases.Overall, the PPP1R15A Polyclonal Antibody (CAB16260) is a valuable tool for researchers investigating the role of PPP1R15A in cellular stress responses and its implications in disease pathology. Its high reactivity and specificity make it a reliable choice for Western blot analysis in a wide range of research applications.
Product Name:
PPP1R15A Rabbit Polyclonal Antibody
SKU:
CAB16260
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human PPP1R15A (NP_055145.3).
cytoplasm, cytosol, endoplasmic reticulum, Golgi apparatus, mitochondrial outer membrane, mitochondrion, protein phosphatase type 1 complex
Calculated MW:
73kDa
Observed MW:
110kDa
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.
Purification Method:
Affinity purification
Gene ID:
23645
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPP1R15A Rabbit pAb (CAB16260) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
