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PPP1R14A Rabbit Polyclonal Antibody (CAB10160)

SKU:
CAB10160
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PPP1R14A Rabbit Polyclonal Antibody
SKU:CAB10160
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
Sequence:MAAQ RLGK RVLS KLQS PSRA RGPG GSPG GLQK RHAR VTVK YDRR ELQR RLDV EKWI DGRL EELY RGME ADMP DEIN IDEL LELE SEEE RSRK IQGL LKSC GKPV EDFI QELL AKLQ GLHR QPGL RQPS PSHD GSLS PLQD RART AHP
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CPI17; CPI-17; PPP1INL; PPP1R14A
Positive Sample:K-562,Mouse lung,Mouse brain,Mouse spleen,Rat lung,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:17kDa
Observed MW:19kDa

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Purification Method:Affinity purification
Gene ID:94274
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using PPP1R14A Rabbit pAb (CAB10160) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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