The PPP1R12B Polyclonal Antibody (CAB17510) is a valuable tool for researchers studying PPP1R12B, a regulatory subunit of protein phosphatase 1 involved in signal transduction and cellular processes. This rabbit polyclonal antibody is highly specific to human samples and is validated for use in Western blot applications. By binding to PPP1R12B, researchers can detect and analyze the protein in various cell types, making it ideal for studies in cell biology and signal transduction pathways.PPP1R12B is known to play a crucial role in controlling actin cytoskeleton dynamics and cell migration, making it a target of interest in cancer research and other diseases involving cell motility.
Understanding the functions and regulation of PPP1R12B is essential for unraveling its role in disease progression and identifying potential therapeutic strategies. The use of the PPP1R12B Polyclonal Antibody in research settings can provide valuable insights into the complexities of cellular signaling pathways and contribute to the development of novel treatment approaches.
Product Name:
PPP1R12B Rabbit Polyclonal Antibody
SKU:
CAB17510
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 660-930 of human PPP1R12B (NP_002472.2).
A band, cytoplasm, cytoskeleton, cytosol, nucleoplasm, Z disc
Calculated MW:
110kDa
Observed MW:
110kDa
Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome.
Purification Method:
Affinity purification
Gene ID:
4660
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPP1R12B Rabbit pAb (CAB17510) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
