PPP1R12A Rabbit Polyclonal Antibody (CAB6700)
- SKU:
- CAB6700
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | PPP1R12A Rabbit Polyclonal Antibody |
SKU: | CAB6700 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 710-970 of human PPP1R12A (NP_002471.1). |
Sequence: | TIGR SRST RTRE QENE EKEK EEKE KQDK EKQE EKKE SETS REDE YKQK YSRT YDET YQRY RPVS TSSS TTPS SSLS TMSS SLYA SSQL NRPN SLVG ITSA YSRG ITKE NERE GEKR EEEK EGED KSQP KSIR ERRR PREK RRST GVSF WTQD SDEN EQEQ QSDT EEGS NKKE TQTD SISR YETS STSA GDRY DSLL GRSG SYSY LEER KPYS SRLE KDDS TDFK KLYE QILA ENEK LKAQ LHDT NMEL TDLK LQLE KATQ R |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | MBS; GUBS; M130; MYPT1; PPP1R12A |
Positive Sample: | HL-60,BT-474,Mouse heart,Rat brain |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm. |
Calculated MW: | 115kDa |
Observed MW: | 130kDa |
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 4659 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |