The PPP1R12A Polyclonal Antibody (CAB6700) is specifically designed for research involving the protein PPP1R12A, a key regulator of smooth muscle contractility and cell motility. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to PPP1R12A, this antibody allows for the detection and analysis of this important protein in various cell types, making it an ideal tool for studies in cell biology, muscle physiology, and cancer research.PPP1R12A, also known as myosin phosphatase target subunit 1, plays a crucial role in the regulation of smooth muscle contraction by dephosphorylating myosin, leading to muscle relaxation.
Dysregulation of PPP1R12A has been implicated in various diseases, including cardiovascular disorders and certain types of cancer. By understanding the function and activity of PPP1R12A, researchers can gain insights into the mechanisms underlying these diseases and develop targeted therapies to combat them.Overall, the PPP1R12A Polyclonal Antibody is an essential tool for researchers looking to investigate the role of PPP1R12A in cellular processes and disease pathways, providing valuable insights that may ultimately lead to the development of novel therapeutic strategies.
Product Name:
PPP1R12A Rabbit Polyclonal Antibody
SKU:
CAB6700
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 710-970 of human PPP1R12A (NP_002471.1).
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPP1R12A Rabbit pAb (CAB6700) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
