The PPP1R12A Polyclonal Antibody (CAB0587) is a valuable tool for researchers studying the protein PPP1R12A, also known as protein phosphatase 1 regulatory subunit 12A. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By specifically targeting and binding to PPP1R12A, this antibody allows for the detection and analysis of this important protein in a variety of cell types.PPP1R12A is involved in the regulation of protein phosphorylation and plays a critical role in various cellular processes, including cell division and migration. Dysregulation of PPP1R12A has been implicated in a number of diseases, making it a promising target for research in areas such as cancer, cardiovascular disease, and neurological disorders.
By understanding the function and regulation of PPP1R12A, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify novel therapeutic targets. Overall, the PPP1R12A Polyclonal Antibody (CAB0587) is a reliable tool for researchers seeking to explore the role of PPP1R12A in cellular processes and disease pathogenesis, ultimately contributing to advancements in the field of biomedical research.
Product Name:
PPP1R12A Rabbit Polyclonal Antibody
SKU:
CAB0587
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MBS; GUBS; M130; MYPT1; PPP1R12A
Positive Sample:
U-87MG,LO2,Jurkat,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
115kDa
Observed MW:
140kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPP1R12A Rabbit pAb (CAB0587) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
