PPP1R12A Rabbit Polyclonal Antibody (CAB0587)
- SKU:
- CAB0587
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | PPP1R12A Rabbit Polyclonal Antibody |
SKU: | CAB0587 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1). |
Sequence: | MKMA DAKQ KRNE QLKR WIGS ETDL EPPV VKRQ KTKV KFDD GAVF LAAC SSGD TDEV LKLL HRGA DINY ANVD GLTA LHQA CIDD NVDM VKFL VENG ANIN QPDN EGWI PLHA AASC GYLD IAEF LIGQ GAHV GAVN SEGD TPLD IAEE EAME ELLQ NEVN RQGV DIEA ARKE EERI MLRD ARQW LNSG HIND VRHA KSGG |
Tested Applications: | WB IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | MBS; GUBS; M130; MYPT1; PPP1R12A |
Positive Sample: | U-87MG,LO2,Jurkat,HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm. |
Calculated MW: | 115kDa |
Observed MW: | 140kDa |
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 4659 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |