The PPOX Polyclonal Antibody (CAB6397) is a valuable tool for researchers studying the enzyme protoporphyrinogen oxidase (PPOX). This antibody, generated in rabbits, is highly specific for human PPOX and has been validated for use in Western blot applications. By binding to PPOX, this antibody allows for the detection and analysis of this important enzyme in a variety of cell types.PPOX is a key enzyme in the heme biosynthesis pathway and plays a critical role in the production of heme, an essential component of hemoglobin and other hemoproteins. Dysregulation of PPOX has been linked to various disorders, including porphyrias, a group of rare metabolic disorders characterized by the buildup of heme precursors.
By studying PPOX expression and activity, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the PPOX Polyclonal Antibody is a valuable tool for researchers interested in studying PPOX and its role in heme biosynthesis and related diseases. Its specificity and sensitivity make it ideal for use in various research applications, including immunohistochemistry and flow cytometry.
Product Name:
PPOX Rabbit Polyclonal Antibody
SKU:
CAB6397
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPOX (NP_001116236.1).
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.
Purification Method:
Affinity purification
Gene ID:
5498
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPOX Rabbit pAb (CAB6397) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
